Business Strategy Of Louis Philippe Marketing Essay

Business Strategy Of Louis Philippe Marketing Essay Art for arts sake makes no more sense than gin for gins sake said by Somerset Maugham.   Finis Coronat Opus  or, quite simply,  The finish crowns the work.Every garment in this line is a suggestive example of this philosophy. The range has subtle design distinctions that will glee the wearer and allow him to discover something new every day. Shirts with fine sewing on inner sleeve plackets, trousers with a special tape on the inner waistband tucked under the trim fabric, suits and jackets with the centre cover button stitched with thread of the brand color to make a subtle design. About Louis Philippe Louis Philippe (extracted from Franco-Italian culture) lures its name and its stimulus from King Louis Philippe of France Louis Philippe. It is an international men’s fashion brand which symbolizes status or rank and lifestyle. Louis Philippe tantamount with premium was launched in India in 1989 with the concept that the person, who wears it, is a man of infallible taste p reference and different class. Louis Philippe always focuses on global fashion that gives it indisputable premium and an exclusive image. Louis Philippe is a leading brand in formal as well as quasi-formal wear. From its commencement, Louis Philippe has been seen as the supplier of fine clothing for the discriminating European gentleman. This international super brand offers stylish ensembles for the fashionable Indian man also. Garments under such label combined the finest fabrics with designs inspired by the latest global trend of fashion and addressing the needs of the style-conscious fashionable male. In India, Louis Philippe garments are available at 119 stores in 50 cities in 21 states, and at the exclusive LP stores created by famous European architect John Marsala. The brand continues to be the leader in retail sales in all leading menswear and department stores. Its retail experience is further amplified by Madura Fashion & Lifestyle’s retail chain – Planet Fa shion. Louis Philippe Products Louis Philippe has launched its sub-brands (LP and Luxure) in November 2007. LP by Louis Philippe aims at young achievers and fashionable urbanites, who demand clothing flexibility. It establishes the style mantra for younger generation.  LP is the brand for those who are in a flurry to make a mark in this world, but will not compromise on either their values.  With two trendy fits in shirts and denims, four styles in trousers and three in suits and jackets, the LP range provides ample options for customizing a wardrobe to individual preferences.  Its product categories are shirts, trousers, suits, jeans, blazers, shoes, T-shirts and accessories. Louis Philippe launched the Luxure brand in select stores across India in November 2008. Created to deliver tailored elegance for the refined aficionado, the masterpiece collection strengthens brand Louis Philippe’s promise to deliver excellence to customers who have an appreciation of the finer t hings in life. With Luxure, consumers have access to exquisite apparel, consummate craftsmanship, luxurious materials and delicate aesthetics, all cores to the values and heritage of Louis Philippe. Business Strategy of Louis Philippe Madura Fashion & Lifestyle, a division of Aditya Birla Nuvo Ltd, is one of India’s fastest growing branded apparel companies and a premium lifestyle player in the retail sector recording a blazing growth rate of over 25 per cent year-on-year. After consolidating its market leadership with its own brands, it introduced premier international labels, enabling Indian consumers to buy the most prestigious global fashion wear and accessories within the country. The company’s brand portfolio includes product lines that range from affordable and mass-market to luxurious, high-end style and cater to every age group, from children and youth to men and women. Madura Fashion & Lifestyle is defined by its brands – Louis Philippe, Van Heusen, Al len Solly, Peter England and People that personify style, attitude, luxury and comfort.

Business Management Research Paper Example | Topics and Well Written Essays - 250 words

Business Management - Research Paper Example The Spain team has the responsibility of expanding the firm’s product into the Latin America and Eastern European marketplaces. The team should take over the marketing function of the company. The Finland team has existing relationship with many European carriers. The Finnish deal included the acquisition of a product line of games. The Finland team should take over the function of supply chain and logistics. 2. Digital Chocolate is a major player in the gaming industry. The exact market share of the company is not provided by the case study, but it is stated that the 10 largest gaming companies (which includes Digital Chocolate) hold 70% of the market share in the gaming industry which is valued at $34 billion. Overall my assessment is that the company has done a good job penetrating the marketplace and is doing well financially. The firm was able to establish deals with 200 phone carriers. The arrival of the iPhone and the subsequent Apple Application store helped Digital Ch ocolate establish a greater presence in the mobile gaming sector. The company was able to obtain 10 million downloads of its titles within the first 100 days of the Apple App store launch. In 2009 Digital Chocolate ranked first among Pokergamer.biz Quality Index.

Personal experience essay Example | Topics and Well Written Essays - 1000 words

Personal experience - Essay Example His other essay, â€Å"Shooting an Elephant,† exposes the evils of imperialism for both local people and white settlers. In The Girl from H.O.P.P.E.R.S., Hernandez emphasizes that despite physical differences, people can find something that will connect them to one another. This writer can connect with the feelings of social inequality in â€Å"Such, Such Were the Joys† and social stratification and division in â€Å"Shooting an Elephant† and The Girl from H.O.P.P.E.R.S., but the same pessimistic Orwellian tone does not dominate her life because of the existence of strong family ties and friendships that helped her grow as a woman and as a human being. Personal experiences of social inequality are similar to these essays and the novel, where social class affected how people treated each other. Growing up as a middle class and studying at a private school provide similar experiences as Orwell’s â€Å"Such, Such Were the Joys.† He experienced being be aten up because of wetting his bed. He could not understand why something he could not control result to the head mistress, Flip, seeing that â€Å"the proper cure was a beating† (Orwell â€Å"Such† 1). The beating inevitably made him more anxious and increased his bed wetting woes. It is possible too that his restlessness comes from the feeling of not being able to fit into a posh school. Sambo wants to attract fame and money to his school: â€Å"One was to attract titled boys to the school, and the other was to train up pupils to win scholarships at public schools, above all at Eton† (Orwell â€Å"Such† 2). In doing so, he increases the gulf between Orwell and his richer classmates. The differences in treatment between the very rich and just affluent enough is clear. Orwell thinks about these differentiated treatments: â€Å"I doubt whether Sambo ever caned any boy whose father's income was much above ?2,000 a year† (Orwell â€Å"Such† 2). This writer feels the same brunt of being poor, although not entirely poor. Having studied in a private school, where many students are extremely rich, she feels left out. The school is not her second home, but a place to be judged and compared to others. Being different to the eyes of others because of physical differences and confidence is compared to The Girl from H.O.P.P.E.R.S. Rena befriends India, who thinks she is a â€Å"little monster† (Hernandez 14). As a midget wrestler, India is both cheered and jeered. In the end, she feels a small and helpless being with no friends and future. The writer shares the same helpless feelings, but not the lack of future. She manages to feel strong despite feeling small in a world that she feels is bigger than her. The writer feels the same negative way of seeing her difference; her strength has become her source of loneliness too. Social division happens because some people focus on differences in race, religion, political ideologie s, and gender. Being a strong and individualistic woman is considered abnormal, as in The Girl from H.O.P.P.E.R.S. Rena has the respect of people because of her size and strength. But like India, society sees her as a freak. This is why she feels empathy for India; they are the same in many ways, only in different packages. This writer understands how it feels to be socially alienated. It cuts into her identity, which could have made her just as miserable as

Critically evaluate the difficulties of assessing malingering in Essay

Critically evaluate the difficulties of assessing malingering in anxiety disorders - Essay Example In view of the basic assumptions that are made about the honesty of self reporting by the patient, the detection of mild forms of malingering becomes difficult. Forensic and other tests have been developed to detect malingering, especially in the case of those individuals whose competency to stand trial for criminal convictions is being tested. Malingering has been defined by the American Psychiatric Association as the â€Å"intentional production of false or grossly exaggerated physical or psychological symptoms† in the pursuit for â€Å"external incentives† (APA, 1994, p 63). Some of these incentives may be escaping criminal conviction, receiving financial compensation or in order to gain access to drugs or medication. In some cases, individuals may exhibit symptoms of malingering solely for the purpose of receiving sympathy and care through being cast in a patient’s role, and in particular may be a component of psychiatric conditions such as anxiety or personality disorders. (APA, 1994:648). Malingering is generally encountered in the case of those individuals for conditions that may be easily faked such as pain or anxiety and is most prevalent in male dominated environments such as the military or in correctional facilities and veteran hospitals. Malingering poses a challenge in health care because it affects the delivery of health care to patients who may genuinely need it by diverting treatment and medical resources to those who are not really ill and by wasting the time and energy of staff members thereby resulting in burnout among nurses and medical personnel.(Resnick, 1998). Malingering on the pretext of anxiety disorder is particularly prevalent among school children. It shows up in the form of refusal to attend school. Evans (2000) conducted a study on malingering in school refusals due to purported anxiety disorders and found that

Please follow the movie and write one paper about this question and Essay

Please follow the movie and write one paper about this question and anlyes - Essay Example The moka is very important because the leader’s reputation and prestige depends on the moka he can organize. Moka is also the manifestation or a validation of the character of the Ongka of his ability to influence and be followed by his tribesmen in raising the gifts for the moka. In the documentary, the Ongka was able to persuade his tribesmen in raising the gifts that will given in the moka for another tribe. Since the moka is an important occasion in the Kawelka tribe to maintain peace and foster trade among other tribes in different valleys, participants in the moka wear their traditional and Highland decoration decorations which typically constitute of the things they have amassed in raising the moka such as pearl shells. The bigger the moka the grander these adornments are because they reflect the reputation and prestige of the Big Man of their tribe who is the Ongka that organized the

International Relations of Global Environmental Change Essay

International Relations of Global Environmental Change - Essay Example It is important to understand however the problems of implementing stern policies. The consequences of these policies on environment will be highly negative and this may decrease international trade drastically. Developing world is lacking the technology to produce environmental friendly products (Matsumura, A. 2010). They are taking initiatives but it will take a lot of time for any major change. In such situation where developing world in lacking resources, the strict policies for environmental protection can cause damage to their economy and to the world as well. Reducing global trade can lead the world into a financial crunch. This is why World Trade Organization cannot take up strict policies regarding global trade. International trade needs to be encouraged in order to maintain the economic prosperity in the world. Countries like South Korea and Taiwan have an export oriented economy. If the global trade is reduced then their economy will suffer badly. Also the world cannot just ban products from the developing world because they are producing high quality cheap products. The same products cannot be produced with similar costs in the developed world.This means that the world economy will also suffer if developing countries are forced to abide by the environmental protection law. People all over the world will have to pay high costs for products and economies of developing countries will suffer as a result of strict restrictions on global trade. This is the reason why global trade cannot be curtailed over environmental concerns. ... Reducing global trade can lead the world into a financial crunch. This is why World Trade Organization cannot take up strict policies regarding global trade. International trade needs to be encouraged in order to maintain the economic prosperity in the world. Countries like South Korea and Taiwan have an export oriented economy. If the global trade is reduced then their economy will suffer badly. Also the world cannot just ban products from the developing world because they are producing high quality cheap products. The same products cannot be produced with similar costs in the developed world. This means that the world economy will also suffer if developing countries are forced to abide by the environmental protection law. People all over the world will have to pay high costs for products and economies of developing countries will suffer as a result of strict restrictions on global trade. This is the reason why global trade cannot be curtailed over environmental concerns. Also devel oping countries are not in favor of environmental protection laws. They argue that the developed world was allowed to use the methods of production and this contributed to their growth and dominance today. Now when the developing world is following in their footsteps then they are being rebuked and stopped. Environmental laws are seen by many as methods by which developed world is trying to hinder the economic growth of developing world. This is another reason why tensions exist between environmental protection and global trade. Developing world sees this as an opportunity to expand its economy while any strict environmental policies will do

IT Project Management Ch7 Assignment Example | Topics and Well Written Essays - 500 words

IT Project Management Ch7 - Assignment Example The main benefit of using its cost management feature is that it allows project managers to manage all the project related information at a single place. For instance, all the project related information such as resources, activities, schedule and costs can be managed in a single application at the single location. As a result, it becomes easy for them to integrate project activities with associated cost and schedule (Schaffhauser, 2010; Microsoft Corporation, 2015; Schwalbe, 2012). Every project manager uses a specific project management tool in order to manage their project management activities. For the cost management, they use specific criteria and some firms have their own software tools that allow them to calculate costs according to their available resources. In some cases, cost estimates are developed and maintained separately from the project plan. Though, MS Project 2010 encompasses all the features that are necessary to maintain a complete project cost management process, but it is still a new component for the majority of project managers, and it can take some time to get complete expertise (Schwalbe, 2012). The project completion opens a door for learning by measuring the performance and improving on the basis of mistakes. To evaluate the performance of any project, the Earned Value Management is quite a significant evaluation practice used in project management (Schwalbe, 2012). A study conducted by Dr. Song that is sponsored by the PMI (Project Management Institute) and CPM (College of Performance Management), is purposed at assisting the project managers to understand and measure the extent of practicing the Earned Value Management technique in project management as well as its practitioners’ base. This study’s important aspect is to deliver a cross sectional perspective of most of the recent EVM practices by surveying more than 600 project management experts. For getting a more useful and practical insight

Trifles deals with gender perception a sensitive reading of the drama Essay

Trifles deals with gender perception a sensitive reading of the drama involves understanding how the men view the women, how t - Essay Example "Trifles" deals with gender perception; a sensitive reading of the drama involves understanding how the men view the women, how the women view the men, and how the women ultimately triumph over the men. Men are presented as an egoistic species that reserve the back roles for the women right from the beginning of the play. Throughout the development of the play, women seem to be placed in the dark and they consequently withdraw themselves to trivial details that men count as unimportant in the case. â€Å"She didn't pay much attention,† are examples of words men use in the play to refer to women (vcu.edu, 6th conversation by Hale). At one point in the play, women are depicted as a species that concentrates on trifles at an instance when men plainly mock them for linking a quilt to murder case evidence. "Trifles" deals with gender perception; a sensitive reading of the drama involves understanding how the men view the women. Other trivial details that the women fall on in their assistance to unravel the murder are equally rejected by the chauvinistic men in the play. In aromatic turn of events, the very rejections termed trivial become the main pieces of reliable evidence.

How to Create a Smash Book Essay Example for Free

How to Create a Smash Book Essay How to create a smash book, not a scrapbook. A smash book is a place where you can place the little things in life’ ticket stubs, magazine clips, recipes, snapshots, love letters, design ideas, pictures, and notes to self. It is faster than scrap booking and is portable too. A smash book can be made from a ringed binder or a note book or you can buy a book from k and company at a craft store or online. They are personal on each page with picture design on each that makes it unique. Before creating your smash book, know the different styles of smash books. There are twelve: simple orange, cutesy, pretty pink, retro, and smart, couture, tasty, nostalgia, doodle red, mod, 360 folio, and eco green. The simple orange smash book pages are not like the others, there a lot more plain, and simple. You can do a lot more with the pages. You can chose your â€Å"theme† easier because of the graphic pages chosen for this book in particular. The Cutesy is as described, very cute looking pages. Each page is filled with cute simple little girly things. Such as flowers or pinks and innocent looking pictures. The pretty pink is very floral and girly almost like the cutesy but different in a way because it has a lot more flower prints in it. The retro blue is designed for a retro look. If you like typography you will love this one. If you can’t help to tap your foot to the beat you will love this one because that is what this smash book is all about. It has a sense of humor to it, and it is my personal favorite. The smart smash book is covered with school inspired images and graphics. In comes in a studious style. It is great for school related things. However, the couture smash book is fun, shiny and very fashion like, with lots of color. It is a lot of girl’s favorites. The Tasty smash book is covered with food inspired pages. Original illustrations and hand lettering design, you can make this in a snap. The nostalgia style is also filled with school filled original illustrations and hand lettering designs. However not as a clean slate of a look as the smart smash book. The Doodle red smash book is filled with an artsy enthusiasm. Mod smash book is a hip style. This smash book is covered with clean lines and patterns. The 360 folio is filled with pages for everyday of the year. Lastly, the Eco green smash book is filled with easy breezy graphics, and nature loving images. Nonetheless you can’t forget when you buy your book, you need to get things/accessories to decorate it. Next, you’ll need to have simple things to decorate your smash book, such as: decorative tape, chipboard pieces, letters, stickers, souvenirs, ribbon, sequins, pockets, envelopes, brads, scissors, glue, scrapbook paper, color pencils, and pens. None the less you will want to use 3d embellishments, alphabet stickers, glitter, stickers maybe by big ideas, paper studio, and k and company. Gemstone are great to use. Border punch, ink pad by color box, distressed ink, and stamp by stampabilities, and patterned paper. After you get the smash book accessories, decorate the cover- it is the best way to decorate the cover by putting your favorite saying on the front using chipboard pieces. You could put what your smash book â€Å"theme† is about if you have one. Or you can just decorate the front cover by doodling on it, if you desire just to do that instead. If you made your own smash book out of a three ring binder, add scrapbook paper to your book on every page. Make it creative. You don’t need to just have one color or design per page. Mix it up and use different sizes and different colors. Don’t worry about this if you bought your smash book though. Meanwhile, you will want to sort out all your stuff into desired categories. You might want to sort them out into categories such as: concerts, journals, notes, travel, friends, family, school, ideas, pictures, clips, whatever it may be, categorizing them will help you when smashing them in your book. Add photos to your page. Use decorative tape and then add your souvenirs. Pockets and envelopes are great for holding big things. Next you want to decorate the page. Don’t over decorate too much. Then it will look overwhelming. But if you under decorate it will look very boring. Very un-creative. Since smash products are the best thing when decorating your smash book; sometimes it’s all you need. Smash captions are ready to stick, all you have to do is write your caption in. Smash flag clips, clips to attach photos, notes and more. Smash pads allow you to voice your opinion or jot something down, then smash it in. Smash date stamp, is exactly what it says a date stamp but with sayings like â€Å"I’m a hot mess†, or â€Å"today is†, â€Å"I love this†! With the date next to it. Smash tape is tape with a design on it, so you can be creative. Smash tape can hold your photos in place or even a feather? Who knows? Next is smash pockets, which are great for holding the bigger things like brochures and things like that. Smash bands, hold your smash book together. And your pens even closer. Now take your desired category you want to smash, your glue and pen and smash it in. Use your date stamp and date it if you would like. Add a smash caption too, some smash tape to hold in a picture that is a memory you want to keep! Some stickers, and writings of your own even drawings will work too if your artistic. Remember, this smash book isn’t a scrapbook. It doesn’t have to be all clean and neat. It’s not just about the pictures. It about the thought, a jot a feather that’s caught, it’s the idea that matters! Everything you have been keeping in that junk drawer for years, let it come out. Have fun with it. Don’t let it hide, and smash it! . It is easy to take along, anywhere you go. It has to time limit. It doesn’t take hours. So smash away and have fun, just remember those steps . Just choose your smash book style, or create your own, have the simple things to create your own, get the smash accessories, sort out all your stuff into categories and then smash it in and decorate your page to your desire.

Features of Java Operating System

Features of Java Operating System Introduction: When 1st java card details were issued, the card chips where 8 bits with a few hundred of bytes of RAM and a few kilo bytes of EEPROM. These sever constraints led the java card forum and Sun to issue a had lots of limitations compared to standard java. This specification does not specify and operating system but an execution environment that most player in the smart card industry is able to implement on top of his own proprietary operating system. The silicon market evolution seems to indicate that next generation products will be based on much more powerful machines (32bits, 64bits, RISC, cache are becoming common in chip category) the smart card industry, through the java card forum, has clearly indentified this evolution and has started initiatives like the java card 3.0 details process that aim to define upcoming version of java card adapted nest era of hardware with this hardware evolution, java has also evolved a lot. It is more limited to the desktop environment and is now present in both the server market with J2EE and in the embedded system market with J2ME. This evolution brought interesting feature to the java platform process for running long lived application are popular on the server side and less java subset applicable to categories of devices has been defined with J2ME configuration. The purpose of this assignment is to have overview of what can be considered now as the less java feature that can be integrated to modern smart card hardware and how these features that can be integrated to modern smart card hardware and how these features can be turned into a card operating system that cope with the smart card industry particular constraint like big production, personalization post issuance. The reason for targeting an operating system instead of an additional software layer like its done in the computer world with standard JDK is to use java as the unique hardware abstraction for applications without intermediate levels to reduce the workload and increase the efficiency. Features of Java Operating System: The java platform defined in different techniques the java VM (Virtual Machine) specification and the java language specification. In the context of operating system these techniques are only relevant at the running environment level. The selection of the java Operating System features is guided by the classical needs of OS. The coding, linking, running engine and memory management for the performance. Class file of Java: The class file is Java define in the JVM (Java Virtual Machine) specification as delivery format for the code of classed to load into a Java VM. This format is considered to be verbose and not compact, but it has some other properties that provide a maximum flexibility both for the development and for the code application update. Other formats such as the Java card 2.x Cap file or the JEFF file format are focusing on reducing file size using the command and techniques like linking information removal or file structure reorganization. These techniques have good result in size reduction but induce deployment constraints depending on the degree of size reduction for example a Cap file can be ten percent size of the equivalent set of class files, but it requires an off card conversion step for the developer and to maintain off card a database of linking information to perform card pre linking which is painful work for deployment and card management. We argue that on card class file processing that provides the same reduction ration as the JEFF conversion fifty percent reduction. The process explaining more detail in figure below. Since the load link operation occurs only once in the life times on the class, we think that the development benefits brought by loading standard class files are worth the effort of loading the class file in its entirety and applying an on board conversion process that enable to store in card memory a structure equivalent to fifty percent of the original class file size. One of the main industrial profits of choosing the class file and defining you own conversion process is that it gives freedom for differentiation most of the card manufacture can choose an internal format adapted to his own particular constraints. Engine Multithreaded Execution: the java platform has native support for multithreaded and synchronization and even in the lowest configuration defined for the embedded system market like CLDC, this help to maintained the one may think that for embedded and resource constrained devices the Support for the multithreading may be too heavy to be reasonable, well there are lots of reason to have multithreading support in the core of very small java operating system. The core reason is that it enables to write power application framework on top of the Operating System, in java without needing complex native modifications of the Operating System and its happen when the SIM Toolkit application model has been explained in java card. There are also lots of classical reasons to use threads that are briefly described in the literature. In limited devices like smart cards, memory such as the Virtual Machine implements must be carefully choose an efficient and resource helping implementation of the multithreading support. The existence of java application only on the top of java operating system collaborating with native code and not with native application like in a desktop computer seems to support a green thread like implementation rather than a native thread implementation. Java Memory Management: One of the interesting feature of Java Virtual Machine (JVM) is the automatically garbage collected heap that is used to allocate java objects when they are created, letting the burden of moving, compacting, freeing the memory to the system instead of the developer. This interesting feature was lacking in the java card specification and give the developer of complex applications to developer their own memory management routines within the application code, which is written in java, this disadvantage of java card to behave as an Operating System. The programs shouldnt handle such sensitive task. There are several types of garbage collectors but the ones that seem the more appropriate to smart card like environments are generational collector. This type of collector are well adapted to server like long lived application which is typically the case of smart card program. Actually the memory manage for the object instances is separate from the memory management of system data like description, threads, code, classes, objects. This normally leading toward the complexity, bigger footprint of the memory management and static segmentation of the memory. We are experimenting an alternative to such a split organization that is to use unified object oriented memory management and static segmentation of the memory. We are experimenting an alternative to such a split organization that is to use a unified object oriented memory manager that both types of programs and system objects, in this case most of the things in the system is an object including java objects instances, loaded, code, classes and every object in the system is subject to garbage collection. This memory management leads to easy management code which is then hopefully smaller and more robust. Different Application Models: Most of the earlier feature of the Java Virtual Machine (JVM) describe above have common objective put in the Operating System the functionality that will make smaller and more efficient application. One great strength of java that was lost in java card and that we think must be reconsidered for next generation smart card Operating System is to be application model independent. The Java Virtual Machine (JVM) doesnt clarify a particular application model, but gives all the core features that are necessary to build various successful frameworks such as applets, serves or RMI Objects. Configurations: The smart card program and system designers and developers have considerations that are very similar to the people working in the mass production embedded system devices market. How to debugging fast/safely the Operating System and the programs, how to build quickly a product configuration, and how to decline and Operating System in product range to leverage the investment. There is more, the smart card industry has his own particularities like for instance the need for personalization of every issued card with the holder information. Development Issues: The use of java is in itself the beginning of a solution for some of these problems, because of the wide range of products freely or commercially available to help designers and developers. But the high safety exigency on smart card products implies that maximum effort must be made on any tools that would allow the developers to operate in an environment as close as possible to the actual device. Debugging the code while it runs. Issues: Product issue and other tools are help in the production and personalize phase of the cards. The ROM intervenes at the production step, to generate the code to mask into the chips ROM. It is complemented by the memory serialize which is used to build memory images saves in files. It will be sent to the card at initialization and personalize time to build the right memory structure that will put the card in the right state. Such tools are usually difficult to write and to maintain, but these tasks can be greatly simplified using the introspection capabilities provided by the unified object oriented memory management introduce above. Integrated Operating System: A solution to minimize the cost of these tools is to build an operating system that includes them from the beginning, in a configurable manner, and that can be declined from the same set of sources in different editions adapted to the targeted device. Such a configurable Operating System is illustrated in the following figure. Feature Categories Developer edition of the Operating System would include any developer features like an embedded debugging interface based on the JPDA standard, a shell based console to administer the Operating System load remove classes, create threads. Such a complete edition would of course run on computer and on emulator only not on real cards. Another declination would be the post issuance enabled edition that would be the high end embeddable version of the Operating System, targeted for high end cards with post issuance facilities with a loader linker. On the other eternity would be the minimal edition, targeted for low end cards, without any post issuance capabilities, that would have a much smaller footprint. Conclusion: In this assignment we have highlighted some features of an embedded java Operating System suitable for next generation smart cards considered as java micro server platforms. Three important java Operating System characteristic have been explained class file format acquisition, multithreaded support and unified memory management. Their implementation has been shown feasible and their usage and advantages has been explained in the context of configurable card platform architecture. Thanks to tools such shell console, debugger, ROMizer and Serialzier, the migration path from a rich platform configuration up to a customized platform configuration has been explained. The overall advantages of this embedded java Operating System architecture is its adaptability for being used, on one hand as full fledge java environment to quickly develop and test programs and on the another hand as an engineering environment for mass production of optimized embedded code hosted by limited devices. Though originally developed in the context smart cards, such architecture could be suitable in any embedded system device in which the java development platform can different from the final Java deployment platform. References: [1] The Java Card Virtual Machine specification 2.1.1. Sun Microsystems http://java.sun.com/products/javacard/ [2] Java 2 Micro Edition, Sun Microsystems http://java.sun.com/j2me/ [3] Java Virtual Machine Specification, Tim Lindholm, Frank Yellin. Sun Microsystems. http://java.sun.com/docs/books/vmspec/index.html [4] Java Language Specification, James Gosling, Bill Joy, Guy Steele, Gilad Bracha. Sun Microsystems. http://java.sun.com/docs/books/jls/index.html [5] The JEFF File Format, J-Consortium http://www.j-consortium.org/jeffwg/index.shtml [5] J2ME Connected Limited Device Configuration http://java.sun.com/products/cldc/ [6] Sim API for Java Card, ETSI TS 143 019 V5.2.0 (2002-03) http://www.etsi.org [7] Concurrent Programming in Java. D. Lea. Sun Microsystems, 1999. ISBN 0-201-31009-0, Addison-Wesley [8] Java Platform Debugger Architecture, Sun Microsystems, http://java.sun.com/j2se/1.3/docs/guide/jpda/index.html Master of Science BITE Innovative Technology 1

Expression and Purification of recombinant Green Fluorescent Protein

Expression and Purification of recombinant Green Fluorescent Protein ABSTRACT: The purpose of this experiment was to determine if a His-6 tagged recombinant form of Green Fluorescent Protein could be expressed in a pRSETA vector of E. Coli. This was determined through multiple procedures beginning with purifying the sample with Ni +2 agarose chromatography which showcased the relative fluorescent activity of the samples, which elution sample two (E2) had approximately 100,592.2 RFU/mg . The yield of total protein was found by use of a Bradford Assay and a standard curve. The purity of the GFP was determined by comparing the intensity of bands that appeared at around 31.4 kDa (the molecular weight of rGFP) to a molecular weight ladder on an SDS-PAGE gel. The Western Blot test, utilizing a nitrocellulous membrane, confirmed the expression of rGFP. The Western Blot confirmed that the correct bands were analyzed in the SDS-PAGE gel which E3 had an estimated purity of 0.4, indicating a yield of approximately 29.4 ug of rGFP for the third Elution (E3) after a total p rotein amount of 49 ug was extrapolated from the Bradford assay. INTRODUCTION: The Green Fluorescent Protein was first isolated from the Aequorea Victoria jellyfish and studied by Osamu Shimomura. In other organisms other than this specific jellyfish, there are fluorescent proteins with more color varieties than just green (Ward). GFP is able to fluoresce due to the formation of a chromophore in its center that resides in a stable beta barrel structure. The interactions between Ser64 and Gly67 on an alpha strand form a five member ring structure and its this structure that contains properties for fluorescence. When ultraviolet light is absorbed at 395nm, an emission wavelength of 510nm is seen as fluorescent green light. The excitation wavelength of 395nm excites the electrons within the protein and raises the energy of the protein. As the protein loses energy, it emits the energy at a wavelength of 510nm. Wild type GFP does not have immensely visible fluorescence, therefore a genetically modified form of GFP, GFPuv, was used in order to better observe fluoresc ent activity. For rGFP to be fully expressed, GFPuvs open reading frame was pRSETA vector, which was cut by a restriction enzyme at sites that complimented the start and stop codons of the His-6 tag sequence. A T7 promoter and Xpress Epitope were also genetically added into the vector with the purpose of simplifying purification. T7 polymerase minds to the T7 promoter and activates the production of GFP, while the Xpress Epitope allows for the binding of a primary antibody. The binding of a primary antibody allows for the binding of a secondary antibody which contains horseradish peroxide, producing bands on the nitrocellulose membrane. (2 pg 351) Histidine-6 tags primary purpose is to aid in the purification of rGFP proteins in affinity chromatography. Histidine has an affinity to bind with transition metal ions, and is why a Nickel+2 agarose chromatography was chosen. When a sample is passed through the Nickel+2 agarose column, the His-6 tags fused with rGFP protein will bind to the column and any proteins not of interest or containments will pass through the column. To elute the bound rGFP proteins from the column, elution buffer containing Imidazole is used and competes with the histidine residues for the binding sties to the Nickel+2 agarose column due to its higher affinity for them. Elutions from the column therefore contain rGFP. (2 pg 359) The purpose of this experiment was to see if a His-6 tagged recombinant form of GFP, from the E. coli strain BL21pLysS, could be expressed and then purified using Ni+2 agarose affinity chromatography technology. If this experiment is successful, an inexpensive and relatively simple method to measure gene expression and locate and track proteins may be at hand. MATERIALS AND METHODS: Bacterial Expression Grow two bacterial cultures: G, containing BL21, DE3, pLysS, and the plasmid pRSETA-GFPuv with the GFP sequence, and V, which is identical to G, but it does not have the GFP sequence. Incubate both cultures at about 37Â °C until OD600 equals 0.5. Transfer 1ml of each culture into separate centrifuge tubes and centrifuge to obtain a pellet. The supernatant from the tubes will be discarded and then label the tubes V0 and G0 and stored at -20Â °C. Induce the rest of the culture with IPTG and allow to grow for 3 hours. After the 3 hours, collect 1ml of each culture and centrifuge. Label the tubes V3 and G3 and were store at -20Â °C. Collect 15 ml of G and centrifuge, and label it as G3-15ml and store at -20Â °C. (1 pg 105) Preparation of Crude Extract After the slow freeze process, add 1 ml of breaking buffer (10mM Tris, pH 8.0; 150mM NaCl) to the frozen bacterial pellet G3-15ml, and pipette up and down until completely dissolved. Then transfer the contents to a centrifuge tube and vortex for 5 minutes and place in a 37Â °C water bath for 10 minutes. Afterwards, incubate in dry air at 37Â °C. Centrifuge the mixture at 4Â °C and transfer the supernatant into a clean centrifuge tube and take a small sample to label as GCE. The remaining supernatant will go through the Nickel+2 agarose column. (1 pg 110) Ni+2 NTA Chromatography Pack a 3ml plastic syringe with a small amount of glass wool to prepare the Ni+2 agarose column. After that, pipet 1 ml of breaking buffer into the syringe to remove any air bubbles. As the breaking buffer is going through the syringe, pour some breaking buffer into the leur-lock and then screw it onto the syringe. Add 1ml of 50% Ni+2 agarose into the column and then open the leur-lock to pack the agarose matrix in the colum. Add 5 ml of breaking buffer subsequently into the column to push the ethanol of the Ni+2 agarose through the column. Then close the leur-lock and apply the crude extract to the column and allow 5-10 minutes for the histidine tags on the rGFP to bind with the Ni+2 agarose beads. Open the leur-lock and collect the non-crude extract in a centrifuge tube labeled W1 for the first wash. Add 0.5ml aliquots of breaking buffer to the column and collect the washes in tubes W2-W10. Add 10 increments of 0.5ml of elution buffer and collect in tubes E1-E10. Then store your GC E, W1-10, and E1-10 samples at -20Â °C. (1 pg 111-2) Bradford Assay Analysis To create a Bradford assay, mix protein sample with water, and then add Bradford reagent dye. Vortex the mixture and then transfer it to the wells of a microtiter dish, so that the absorbance of your protein sample can be determined at 595nm using a spectrophotometer. To determine the amount of total protein present in the sample volume, you must create a Bradford standard curve using 0, 2.5, 5, 10, 15, and 20 ug of BSA and the absorbance values observed to determine a best-fit line. Perform the assay in singlicate using samples W1-W6 and E1-E6 to determine if the microplate data falls within the standard curve. Once you have determined what volume of sample to use, repeat the Bradford assays two more times for each sample. Use the standard curve and experimental absorbance values to extrapolate the total protein present in the volume of sample you use. (1 pg 124-6) SDS-PAGE/Coomassie Blue Analysis of rGFP Fractions Create a 12% Resolving Gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x resolving buffer [0.75M Tris pH8.8, 0.4%SDS]. Pour the resolving gel into a gel electrophoresis set up and overlay with some water. Allow the resolving gel to polymerize and proceed to making a 5% Stacking gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x stacking buffer. Pour the stacking gel on top of the resolving gel and immediately insert a comb to form the wells. Prepare your loading samples with 4x sample loading buffer and then vortex, boil, and centrifuge. Do this for the G0, G3, GCE, W2, W3, E2, and E3 samples (or fractions that contained the most rGFP fluorescence). (1pg 111-2) After the gel has solidified, transfer the apparatus into the electrophoresis tank and load the electrophoresis buffer into the tank. Then load your samples into lanes one through seven respectively along with a marking ladder to compare the distances the samples traveled after electrophoresis. Electrophorese at 200V for approximately 45 minutes and then stain your gel with Coomassie Blue dye. To remove the stain that has not been absorbed by proteins, the gel is soaked in dilute acid and methanol. Western Blot Develop an SDS-PAGE gel as previously described using your sample fractions and transfer the proteins onto nitrocellulose transfer membrane using two locking cassette lids with sponges and filter paper encompassing the gel and nitrocellulose. Remove the nitrocellulose and stain the membrane with Ponceau S stain, and allow to incubate for approximately 2 minutes. Once stained, wash the membrane with nanopure water until bands are visible on the membrane. Next, perform the blocking step by placing the membrane in a container containing 5% non-fat dry milk/TBS solution and incubate on a shaking platform. Pour out the blocking solution and add 0.05% Tween 20/TBS solution and then allow it to incubate on a shaking platform, and then pour out the solution. Repeat this wash step two more times. Add mouse IgG anti-Xpress epitope MAb and allow incubate on a shaking platform this is the primary probe step. Then repeat the wash step as mentioned previously three more times, and add Sheep IgG a nti-mouse IgG conjugated horse radish peroxidase polyclonal anti-serum solution (the secondary probe). Allow the membrane to incubate, and then perform the wash step twice. For the final wash, use only TBS, and then add TMB substrate solution and incubate until appropriate banding is shown. Stop the reaction with distilled water and scan or photograph the membrane as the results may fade with time. RESULTS: In the specific strain of E. coli, BL21pLysS, pRSET-GFP represses the lac promoter which is part of the operon needed for T7 polymerase activity. T7 polymerase binds to the T7 promotes of pRSET-GFP and activates the His-6 tag that expresses the fluorescence of GFP. Even though some GFP is produced this way, it is experimentally not enough, so IPTG is added to repress the repressor so an increase of GFP production can occur. V0, V3, G0, and G3 were all bacterial cultures of E. coli with specific distinctions between all of them. V0 and G0 were collected from bacterial cultures that were not yet induced, but G0 contained the GFP sequence while V0 did not. V3 and G3 were the V0 and G0 samples after induction had occurred for 3 hours, and again G0 contained the GFP sequence while V0 did not. W1-W10 were washes collected from the Ni+2 agarose column using the breaking buffer whereas E1-E10 were washes collected using elution buffer. Figure 2. SDS-PAGE/Coomassie Blue stained gel analysis of rGFP SDS-PAGE gel, which was used to analyze our purification of rGFP from a crude extract, and the corresponding molecular weight. This gel was made with a 12% resolving gel and a 5% stacking gel. Wildtype rGFP has a molecular weight for 27kDa. The ladder has kDa rungs of 97.4, 66.2, 45.0, 31, 21.5, and 14.4. The band for rGFP has been indicated on the figure. The different samples were taken during our purification. The highest protein containing washes and elutions were used in the SDS-PAGE gel. G0 represents a sample containing rGFP prior to induction. G3 represents a sample containing rGFP after 3 hours of induction. GCE represents the GFP crude extract after the slow-freeze/quick-thaw process. Figure 3. Western Blot of rGFP We used the Western Blot to detect, and more importantly, quantify proteins that react with antibodies. The Ponceau S stain was performed to highlight the existence of our proteins successfully being transferred from the SDS-PAGE gel. We then performed a blocking step with 5% non-fat dry milk/TBS solution followed by washes with 0.05% Tween20/TBS. The probe first used was mouse IgG anti-Xpress epitope MAb solution, which we followed with washes of Tween20/TBS solution. The second probe used was sheep IgG anti-mouse IgG conjugated horseradish peroxidase polyclonal anti-serum solution, which we followed with two washes of Tween 20/TBS solution with the third wash being pure TBS. Then, TMB solution was added to develop the nitrocellulose membrane and this reaction was stopped by pouring distilled water over the membrane. Above are the 8 lanes, G0, G3, GCE, W2, W3, E3, E4, and a known molecular weight ladder, respectively. CONCLUSION/DISCUSSION: A great amount of research has been put into the field of monitoring gene expression and there are currently many research laboratories that use labeled antibodies and other various means to do so. However, this new recombination technology, a vector can be created to provide a simpler and less expensive approach that can even be performed in vivo. The purpose of this experiment was fulfilled as His-6 tagged rGFP from a particular E. coli strain was successfully expressed, purified, qualified, and quantified by means of Ni+2 agarose chromatography. GFP was initially expressed as a histidine tagged protein that was inserted in E. coli and subsequently a crude extract of this protein was isolated. Throughout the experiment, we were able to inspect and measure the fluorescing activity of our elutions with the use of handheld UV lights. GFP was successfully expressed because of the fluorescent activity that was emitted when shown under UV light. This rGFP was purified using a Ni+2 agarose column which allowed for the binding of the His-6 tagged rGFP to the Ni+2 agarose beads in the column, and subsequently let all other proteins not of interest and containments to be collected in washes. Then, rGFP proteins were eluted by passing elution buffer containing Imidazole through the column because Imidazole has a higher affinity for the Ni+2 agarose beads than does Histidine. Therefore, one could expect that most if not all of the rGFP would be found in the elution washes and would present higher fluorescing activity than the washes with breaking buffer. Figure 1 clearly supports this argument. From referencing Figure 1, the most rGFP was found in E2 because it exhibited the highest fluorescent activity in RFUs. Using a standard curve based off of known quantities of mass of a certain protein, one is able to extrapolate the amount of protein present in ones sample. This can be used to estimate the amount of rGFP activity. For instance, E2s specific activity was found to be approximately 100,592.2 RFU/mg. W1-W6 had the least amount of fluorescing activity because the washes were done prior to the addition of Imidazole through the column. This is expected as Imidazole would flush out rGFP in the elutions, which would produce the highest GFP fluorescing activity. Percent purity of the rGFP samples were approximated using the SDS-PAGE gel with Coomassie blue staining to allow visible bands to appear which were used for comparisons. The molecular mass from each individual sample was determined by comparing the bands with the bands of the known weight ladder. The percent purity of each individual band was determined by comparing the bands against each other. The molecular weight of wild type rGFP is 27 kDa, and the molecular weight of rGFP for mutant rGFP used for this experiment was approximately 31.4 kDa with the 40 extra amino acids that were added taken into account. For example, E3 percentage of purity was found to be .4. The amount of protein yielded was 29.4 ug of rGFP for the third Elution fraction (E3) after a total protein amount of 49 ug The Western Blotting technique detected and quantified proteins that reacted with a specific antibody. In our case, it verified rGFP was expressed and whether or not it fell in the range of the expected molecular weight of 31.51 kDa. Lanes GCE, G0, G3, and E3 all contained a bright band around 31.4 kDa, indicating that rGFP was existent. This result corresponds to the expected of E3 having the one of the highest rGFP activity. The fact I had used E3 for the SDS-PAGE and Western Blotting skewed my results, but E2 and E3 had the highest activity for me. Even with my error, GFP was effectively expressed and purified and its finding is important to science because it can be used to track cancer causing cells one day to help figure out where problem spots begin and can help eliminate the issue before it escalates indicating that fluorescent proteins might eventually be clinically useful in cancer patients (Hoffman). This can occur since GFP can successfully be expressed in other organisms as this experiment through the use of E.coli, humans cells can certainly be tagged and label the tumour before treatment and then monitor for fluorescence after treatment to identify possible recurrence or metastases (Hoffman). The same can be said with heart disease and other health related problems. The future of GFP remains limitless when scientists can unlock its full medical potential. REFERENCE/BIBLIOGRAPHY (1) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Manual. The University of Texas at Dallas. Richardson, TX. (2) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Lecture Notes. The University of Texas at Dallas. Richardson, TX. (3) Ward, William. History of GFP and GFP Antibodies. Brighter Ideas. 2009. 26 Oct, 2010. http://www.brighterideasinc.com/proteins-antibodies/history-of-gfp-and-gfp-antibodies/ (4) Ninfa, Alexander J. and David P. Ballou (1998). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. (5) Hoffman, Robert M. Uses of Fluorescent Proteins to Visualize Cancer In Vivo: Can Fluorescent Proteins be Used in Humans? 2005. 28 Oct. 2010. http://www.medscape.com/viewarticle/513975_6

Becoming A Professional Chef Essay example -- Career Essays

  Ã‚  Ã‚  Ã‚  Ã‚  The chefs who have made the greatest impression know that their successes depend upon several factors, some of which are inherent; some which are diligently cultivated. Becoming a professional chef not only requires a thorough understanding in food preparation, but many other important points as well. Auguste Escoffier, the originator of modern foodservice wrote; "No theories, no formulae, no recipe, no matter how well written, can take the place of experience." (1)   Ã‚  Ã‚  Ã‚  Ã‚  The formal educational process must begin with the choice of institution, in the United States, the Culinary Institute of America, New York is the leader in traditional culinary cuisine preparation. The curriculum is taught on the Escoffier ideal. The California culinary Academy, San Francisco, also offers culinary education, but along with the New England culinary Institute, Montpelier, Vt, offer an education for occupational demand and placement. In order to gain a solid understanding in basic and advanced culinary techniques, any aspiring chef will find formal training at an accredited school an excellent beginning.An apprenticeship and or on-the-job training can be useful for some individuals in the place of formal education, and is a a necessary follow-up for all chefs in training. Learning should be an ongoing process, there is no substitute for experience;only with practice will classroom teory become fully developed.   Ã‚  Ã‚  Ã‚  Ã‚  The purpose behind the education is learning basic food preparation, learning the styles of knife cuts and food presentation. Most importantly of all food preparation is sanitation.   Ã‚  Ã‚  Ã‚  Ã‚  The importance of sanitation cannot be over emphasized. In a business based upon service and hospitality, reputation and indeed, livelihoods are dependent upon the customers good will.   Ã‚  Ã‚  Ã‚  Ã‚  Food-borne illnesses fall into two categories, intoxicant and infections. An understanding of the causes and preventions will limit any contaminations. The food preparation process emcompresses the sanitation process from the beginning, using properly sterile utincels, food storage, etc.   Ã‚  Ã‚  Ã‚  Ã‚  Being a professional chef like any profession has a great many sides. The career opportunities for a chef that come to mind are that, a chef, a cook. The Bureau of Labor Statistics, Monthly Labor review,November, 1995. By the year 2000, there will be an estimated 16-38% increase for chefs. T... ...ublic, organizing the staff to promote customer relations. The chef must also operate the kitchen, after all that is his profession. The chef must develop the needs and specifications of the kitchen, choose purveyors, organize delivery schedules,make inventory requirements. All the ingredients, no pun intended, will become necessary in a profitable on going operation.   Ã‚  Ã‚  Ã‚  Ã‚  When deciding to be a professional chef; the preferred working environment of the individual will dictate how they wish to apply the skill. Being a chef can lead to other occupations as well, some which are not directly related to the foodservice industry. Teaching the skills of a culinarian, newspaper columnist or journalist critic. There are many possibilities to a professional chef outside a kitchen environment, learning by contact with other professionals is what professionalism is all about.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Works cited The California Culinary Academy,WWW.Baychef.com/educational/ index.html. Website. The Wall Street journal,January 2, 1990, Page 1. The Culinary Institute of America,The New Professional Chef. 5th ed.New York: Van Nostrand Reinhold,1991 Becoming A Professional Chef Essay example -- Career Essays   Ã‚  Ã‚  Ã‚  Ã‚  The chefs who have made the greatest impression know that their successes depend upon several factors, some of which are inherent; some which are diligently cultivated. Becoming a professional chef not only requires a thorough understanding in food preparation, but many other important points as well. Auguste Escoffier, the originator of modern foodservice wrote; "No theories, no formulae, no recipe, no matter how well written, can take the place of experience." (1)   Ã‚  Ã‚  Ã‚  Ã‚  The formal educational process must begin with the choice of institution, in the United States, the Culinary Institute of America, New York is the leader in traditional culinary cuisine preparation. The curriculum is taught on the Escoffier ideal. The California culinary Academy, San Francisco, also offers culinary education, but along with the New England culinary Institute, Montpelier, Vt, offer an education for occupational demand and placement. In order to gain a solid understanding in basic and advanced culinary techniques, any aspiring chef will find formal training at an accredited school an excellent beginning.An apprenticeship and or on-the-job training can be useful for some individuals in the place of formal education, and is a a necessary follow-up for all chefs in training. Learning should be an ongoing process, there is no substitute for experience;only with practice will classroom teory become fully developed.   Ã‚  Ã‚  Ã‚  Ã‚  The purpose behind the education is learning basic food preparation, learning the styles of knife cuts and food presentation. Most importantly of all food preparation is sanitation.   Ã‚  Ã‚  Ã‚  Ã‚  The importance of sanitation cannot be over emphasized. In a business based upon service and hospitality, reputation and indeed, livelihoods are dependent upon the customers good will.   Ã‚  Ã‚  Ã‚  Ã‚  Food-borne illnesses fall into two categories, intoxicant and infections. An understanding of the causes and preventions will limit any contaminations. The food preparation process emcompresses the sanitation process from the beginning, using properly sterile utincels, food storage, etc.   Ã‚  Ã‚  Ã‚  Ã‚  Being a professional chef like any profession has a great many sides. The career opportunities for a chef that come to mind are that, a chef, a cook. The Bureau of Labor Statistics, Monthly Labor review,November, 1995. By the year 2000, there will be an estimated 16-38% increase for chefs. T... ...ublic, organizing the staff to promote customer relations. The chef must also operate the kitchen, after all that is his profession. The chef must develop the needs and specifications of the kitchen, choose purveyors, organize delivery schedules,make inventory requirements. All the ingredients, no pun intended, will become necessary in a profitable on going operation.   Ã‚  Ã‚  Ã‚  Ã‚  When deciding to be a professional chef; the preferred working environment of the individual will dictate how they wish to apply the skill. Being a chef can lead to other occupations as well, some which are not directly related to the foodservice industry. Teaching the skills of a culinarian, newspaper columnist or journalist critic. There are many possibilities to a professional chef outside a kitchen environment, learning by contact with other professionals is what professionalism is all about.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Works cited The California Culinary Academy,WWW.Baychef.com/educational/ index.html. Website. The Wall Street journal,January 2, 1990, Page 1. The Culinary Institute of America,The New Professional Chef. 5th ed.New York: Van Nostrand Reinhold,1991

The Great Expectations of Human Nature Essay -- Great Expectations Es

The Great Expectations of Human Nature Charles Dickens used Great Expectations as a forum for presenting his views of human nature. This essay will explore friendship, generosity, love, cruelty and other aspects of human nature presented by Dickens over 100 years ago. Friendship was one of the human characteristics Dickens enjoyed. Herbert was a true friend to Pip. Moving to London would have been stressful if Herbert had not eased Pip's transition into the city. Herbert informed Pip of Miss Havisham's story when no one else would tell it. When Magwitch arrived and mortified Pip, Herbert was there discuss what was to be done. Herbert was an integral part in the design and implementation of Magwitch's escape. Herbert even saved Pip's life from Orlick's powerful grasp. By associating fellowship with good characters and deeds, Dickens made it known that he admired friendships. Another human characteristic which Dickens appreciated was generosity. This trait appeared in Magwitch and Pip. Magwitch , the starved convict, was forever grateful to Pip for bringing him food in the opening of the book. Magwitch repaid Pip's kindness by opening a door to the young man's dreams. Pip suspected his dear friend, Herbert, would never become prosperous so Pip took some of the money he was receiving from Magwitch and used it as a stipend in Herbert's behalf. By doing this, Pip precipitated Herbert becoming a partner in a prosperous business. Having characters donate money and benefit from it, Dickens conveyed the goodness of generosity. To Dickens, the most noble of human characte... ...me to visit Pip in London, Pip was embarrassed to know him. If Pip "could have kept him away by paying money, [Pip] certainly would have paid money." After years of Joe's friendship and loving care, Pip thought of paying him not to visit. At the end of the novel, Pip learned what an unappreciative person he had been to Joe and asked his forgiveness. Dickens presented this piece of human nature well enough to create sympathy for Joe and all parents who receive ingratitude from their children. Dickens used his characters to convey his thoughts of human nature - good and bad. Dickens believed if he could present both sides of humanity to the public, people would try to better themselves. Dickens hated the Victorian society in which he was bound, and he turned to the pen to alter his bete noire.